Clinical 🅱iochemistry

Rate (kinetic) Reactions:
===================
If the analyte is an enzyme, a molecule which can catalyze the conversion of unlimited numbers of reagent molecules (termed substrates) to product, the amount of product at the endpoint would not reflect the amount of enzyme. Instead, the endpoint would reflect the amount of substrate that was present.
For this reason, enzyme activity is determined by a rate reaction rather than an endpoint reaction. In such cases determination of the enzyme concentration is based on how fast a fxed amount of substrate is converted to
product. The more enzyme present, the faster the conversion.
Examples of enzymes that are often measured in the clinical laboratory include lipase and alanine.

Rate reactions may also be used for measurement of analytes that are not enzymes. For example, if a reaction is very slow to reach an endpoint, a rate method may be more practical in order to obtain a result in a shorter timeframe. Some examples of analytes other than enzymes that are measured using rate reaction include ammonia (a waste product of protein metabolism) and amikacin (a therapeutic drug).

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11.6K viewsOct 6, 2017 at 15:32

Rate (kinetic) Reactions:
===================
If the analyte is an enzyme, a molecule which can catalyze the conversion of unlimited numbers of reagent molecules (termed substrates) to product, the amount of product at the endpoint would not reflect the amount of enzyme. Instead, the endpoint would reflect the amount of substrate that was present.
For this reason, enzyme activity is determined by a rate reaction rather than an endpoint reaction. In such cases determination of the enzyme concentration is based on how fast a fxed amount of substrate is converted to
product. The more enzyme present, the faster the conversion.
Examples of enzymes that are often measured in the clinical laboratory include lipase and alanine.

Rate reactions may also be used for measurement of analytes that are not enzymes. For example, if a reaction is very slow to reach an endpoint, a rate method may be more practical in order to obtain a result in a shorter timeframe. Some examples of analytes other than enzymes that are measured using rate reaction include ammonia (a waste product of protein metabolism) and amikacin (a therapeutic drug).

https://www.tg-me.com/joinchat-AAAAAECuqoTkgQkHpiHlGA

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